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1x phosphate buffered saline  (Thermo Fisher)


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    Thermo Fisher 1x phosphate buffered saline
    1x Phosphate Buffered Saline, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphate+buffered+saline/Phosphate-buffered+saline+(PBS%2C+1X)%2C+sterile-filtered/pmc13088972-371-15-21
    Average 99 stars, based on 1 article reviews
    1x phosphate buffered saline - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Saline:

    Article Title: Glycosaminoglycan-functionalized hydrogels for sustained delivery of tissue inhibitor of metalloproteinase-3 mediating matrix metalloprotease inhibition and extracellular matrix stabilization
    Article Snippet: .. The biomaterials were fabricated by combining 55 μL of 10 % (w/w) GelMA, preheated to 37 °C, with 3 μL of phosphate-buffered saline (PBS, pH 7.3, calcium- and magnesium-free; Gibco, Thermo Fisher Scientific, Dreieich, Germany) and 4 μL of 10 mg/mL LAP. .. The biomaterials were fabricated by combining 55 μL of 10 % (w/w) GelMA, preheated to 37 °C, with 3 μL of phosphate-buffered saline (PBS, pH 7.3, calcium- and magnesium-free; Gibco, Thermo Fisher Scientific, Dreieich, Germany) and 4 μL of 10 mg/mL LAP.

    Article Title: Multiplexed Detection of Membranous Nephropathy Antigens by Multi-Reaction Monitoring Mass Spectrometry
    Article Snippet: .. Frozen kidney biopsy tissue remnants were washed in phosphate buffered saline, followed by bead-beating in 400 μl Pierce IP lysis buffer (Thermo Scientific #87787) containing protease and phosphatase inhibitors (Halt protease/phosphatase inhibitor at 1:100 dilution, Thermo Scientific #87786) to produce protein extracts. .. Protein extracts were immunoprecipitated with protein A/G beads (Pierce #88803) in the presence of 50 ng stable isotope-labeled universal monoclonal antibody (SILuMab K1;Sigma-Aldrich #MSQC6), and 50 ng SILuMab K4 (Sigma-Aldrich #MSQC7).

    Article Title: Sex-dependent impact of Il6 deficiency in Parkinson’s disease mice
    Article Snippet: .. After washing with phosphate-buffered saline, the slices were incubated with Alexa Fluor-conjugated secondary antibodies (Alexa Fluor 488 and 594, 1:1000; Invitrogen, USA) at 37 °C for 1 h. Images were acquired using a Nikon AIR-MP confocal microscope (Japan). ..

    Article Title: Human Milk MiRNA Acts on Infections in Both the Maternal Body and the Child During Breastfeeding.
    Article Snippet: Multicolour flow cytometry was conducted on each HM cell fraction sample to examine the percentage contribution of leukocyte subsets to total milk cells through combined fluorescent-labeled antibodies against CD45-FITC, CD3-PE, CD4-APC, CD8-APC, CD56APC, CD19-PE, and CD14-APC (BD Biosciences, San Diego, CA, USA) (Miltenyi Biotec, Bergisch Gladbach, Germany) as described previously [6]. .. Briefly, freshly isolated and PBS-washed HM cells were further washed with 2% fetal bovine serum (FBS) (Gibco, NY, USA) in phosphate-buffered saline (PBS, Gibco, Foster, CA, USA) and were distributed into sterile 1.5 mL tubes. ..

    Article Title: Robotic Centrifugal Microfluidics with In-Rotation Liquid Supply for the Extraction of Multiple Liquid Biopsy Analytes in One Platform.
    Article Snippet: Alignment of the laser and concentration calibration were performed using proprietary QC control beads (NanoFCM), and size calibration was achieved using silica nanospheres (68–155 nm, NanoFCM). .. Dulbecco’s phosphate-buffered saline (Gibco 1× DPBS, Thermo Fisher Scientific, Waltham, MA, USA) was used as the diluent and blank control. ..

    Article Title: Reduced serum interleukin-2 associates with higher motor severity and along with CD4 T cell alterations may be an early event in isolated REM Sleep Behaviour Disorder
    Article Snippet: PBMCs were isolated from whole blood following the SepMate manufacturer instructions (STEMCELL Technologies). .. Specifically, whole blood was gently mixed with an equal volume of phosphate-buffered saline (PBS, Thermo Fisher) containing 2% heat inactivated low endotoxin qualified fetal bovine serum (FBS, Thermo Fisher) in a 50 mL tube. .. Diluted blood was then carefully transferred to a 50 mL SepMate tube (STEMCELL Technologies) containing 17 mL of density gradient medium.

    Article Title: Dissecting the propensity of RIM1 subdomains to form phase condensates.
    Article Snippet: .. Immunocytochemistry of HEK293T cells and primary neurons Fixed HEK293T cells and neurons transfected with Flag-RIM1 or Myc-RIM-BP2 and seeded on coverslips were permeabilized with 0.3% Triton X-100 (Sigma-Aldrich) in Dulbecco’s phosphate buffered saline (DPBS, Gibco, Thermo Fisher Scientific) for 10 min at RT, and incubated with primary antibodies against RIM1 (1:2000, rabbit, Cat. No. 140013, Synaptic Systems) and Flag-tag (1:1000, mouse, Cat. No. F1804, Sigma-Aldrich), or Myc-tag (1:200, mouse, Cat. No. ab32, Abcam) diluted in 0.1% Triton X-100 overnight at 4 °C. .. The next day, cells were washed with DPBS and subsequently incubated with the corresponding fluorescent secondary antibodies (1:400; Alexa Fluor 568 goat anti-rabbit (Cat. No. A11011, Invitrogen) and Alexa Fluor 488 goat anti-mouse (Cat. No. A11001, Invitrogen) were used) diluted in 0.1% Triton X-100 for 2 h at RT.

    Article Title: Polyplex of peptide-mannan and RNA for intranasal delivery of TGF-β siRNA in treatment of pulmonary fibrosis
    Article Snippet: GAPDH reverse and forward primers were purchased from Integrated DNA Technologies, Inc (Coralville, IA). .. Zeba Spin Desalting Columns (7k MOCO), RiboGreen RNA Assay Kit, PowerUp SYBR Green Master Mix, High-Capacity cDNA Reverse Transcription Kit, DMEM/F-12, CHCA MALDI Matrix, and fetal bovine serum (FBS), Alexa Fluor 647 Dye (AF647), phosphate-buffered saline (PBS, pH = 7.4) were purchased from ThermoFisher Scientific (Waltham, MA). .. TGF-β siRNA and Cy5-labeled TGF-β siRNA were purchased from Horizon discovery (Cambridge, UK).

    Lysis:

    Article Title: Multiplexed Detection of Membranous Nephropathy Antigens by Multi-Reaction Monitoring Mass Spectrometry
    Article Snippet: .. Frozen kidney biopsy tissue remnants were washed in phosphate buffered saline, followed by bead-beating in 400 μl Pierce IP lysis buffer (Thermo Scientific #87787) containing protease and phosphatase inhibitors (Halt protease/phosphatase inhibitor at 1:100 dilution, Thermo Scientific #87786) to produce protein extracts. .. Protein extracts were immunoprecipitated with protein A/G beads (Pierce #88803) in the presence of 50 ng stable isotope-labeled universal monoclonal antibody (SILuMab K1;Sigma-Aldrich #MSQC6), and 50 ng SILuMab K4 (Sigma-Aldrich #MSQC7).

    Incubation:

    Article Title: Sex-dependent impact of Il6 deficiency in Parkinson’s disease mice
    Article Snippet: .. After washing with phosphate-buffered saline, the slices were incubated with Alexa Fluor-conjugated secondary antibodies (Alexa Fluor 488 and 594, 1:1000; Invitrogen, USA) at 37 °C for 1 h. Images were acquired using a Nikon AIR-MP confocal microscope (Japan). ..

    Article Title: Dissecting the propensity of RIM1 subdomains to form phase condensates.
    Article Snippet: .. Immunocytochemistry of HEK293T cells and primary neurons Fixed HEK293T cells and neurons transfected with Flag-RIM1 or Myc-RIM-BP2 and seeded on coverslips were permeabilized with 0.3% Triton X-100 (Sigma-Aldrich) in Dulbecco’s phosphate buffered saline (DPBS, Gibco, Thermo Fisher Scientific) for 10 min at RT, and incubated with primary antibodies against RIM1 (1:2000, rabbit, Cat. No. 140013, Synaptic Systems) and Flag-tag (1:1000, mouse, Cat. No. F1804, Sigma-Aldrich), or Myc-tag (1:200, mouse, Cat. No. ab32, Abcam) diluted in 0.1% Triton X-100 overnight at 4 °C. .. The next day, cells were washed with DPBS and subsequently incubated with the corresponding fluorescent secondary antibodies (1:400; Alexa Fluor 568 goat anti-rabbit (Cat. No. A11011, Invitrogen) and Alexa Fluor 488 goat anti-mouse (Cat. No. A11001, Invitrogen) were used) diluted in 0.1% Triton X-100 for 2 h at RT.

    Microscopy:

    Article Title: Sex-dependent impact of Il6 deficiency in Parkinson’s disease mice
    Article Snippet: .. After washing with phosphate-buffered saline, the slices were incubated with Alexa Fluor-conjugated secondary antibodies (Alexa Fluor 488 and 594, 1:1000; Invitrogen, USA) at 37 °C for 1 h. Images were acquired using a Nikon AIR-MP confocal microscope (Japan). ..

    Isolation:

    Article Title: Human Milk MiRNA Acts on Infections in Both the Maternal Body and the Child During Breastfeeding.
    Article Snippet: Multicolour flow cytometry was conducted on each HM cell fraction sample to examine the percentage contribution of leukocyte subsets to total milk cells through combined fluorescent-labeled antibodies against CD45-FITC, CD3-PE, CD4-APC, CD8-APC, CD56APC, CD19-PE, and CD14-APC (BD Biosciences, San Diego, CA, USA) (Miltenyi Biotec, Bergisch Gladbach, Germany) as described previously [6]. .. Briefly, freshly isolated and PBS-washed HM cells were further washed with 2% fetal bovine serum (FBS) (Gibco, NY, USA) in phosphate-buffered saline (PBS, Gibco, Foster, CA, USA) and were distributed into sterile 1.5 mL tubes. ..

    Sterility:

    Article Title: Human Milk MiRNA Acts on Infections in Both the Maternal Body and the Child During Breastfeeding.
    Article Snippet: Multicolour flow cytometry was conducted on each HM cell fraction sample to examine the percentage contribution of leukocyte subsets to total milk cells through combined fluorescent-labeled antibodies against CD45-FITC, CD3-PE, CD4-APC, CD8-APC, CD56APC, CD19-PE, and CD14-APC (BD Biosciences, San Diego, CA, USA) (Miltenyi Biotec, Bergisch Gladbach, Germany) as described previously [6]. .. Briefly, freshly isolated and PBS-washed HM cells were further washed with 2% fetal bovine serum (FBS) (Gibco, NY, USA) in phosphate-buffered saline (PBS, Gibco, Foster, CA, USA) and were distributed into sterile 1.5 mL tubes. ..

    Control:

    Article Title: Robotic Centrifugal Microfluidics with In-Rotation Liquid Supply for the Extraction of Multiple Liquid Biopsy Analytes in One Platform.
    Article Snippet: Alignment of the laser and concentration calibration were performed using proprietary QC control beads (NanoFCM), and size calibration was achieved using silica nanospheres (68–155 nm, NanoFCM). .. Dulbecco’s phosphate-buffered saline (Gibco 1× DPBS, Thermo Fisher Scientific, Waltham, MA, USA) was used as the diluent and blank control. ..

    Immunocytochemistry:

    Article Title: Dissecting the propensity of RIM1 subdomains to form phase condensates.
    Article Snippet: .. Immunocytochemistry of HEK293T cells and primary neurons Fixed HEK293T cells and neurons transfected with Flag-RIM1 or Myc-RIM-BP2 and seeded on coverslips were permeabilized with 0.3% Triton X-100 (Sigma-Aldrich) in Dulbecco’s phosphate buffered saline (DPBS, Gibco, Thermo Fisher Scientific) for 10 min at RT, and incubated with primary antibodies against RIM1 (1:2000, rabbit, Cat. No. 140013, Synaptic Systems) and Flag-tag (1:1000, mouse, Cat. No. F1804, Sigma-Aldrich), or Myc-tag (1:200, mouse, Cat. No. ab32, Abcam) diluted in 0.1% Triton X-100 overnight at 4 °C. .. The next day, cells were washed with DPBS and subsequently incubated with the corresponding fluorescent secondary antibodies (1:400; Alexa Fluor 568 goat anti-rabbit (Cat. No. A11011, Invitrogen) and Alexa Fluor 488 goat anti-mouse (Cat. No. A11001, Invitrogen) were used) diluted in 0.1% Triton X-100 for 2 h at RT.

    Transfection:

    Article Title: Dissecting the propensity of RIM1 subdomains to form phase condensates.
    Article Snippet: .. Immunocytochemistry of HEK293T cells and primary neurons Fixed HEK293T cells and neurons transfected with Flag-RIM1 or Myc-RIM-BP2 and seeded on coverslips were permeabilized with 0.3% Triton X-100 (Sigma-Aldrich) in Dulbecco’s phosphate buffered saline (DPBS, Gibco, Thermo Fisher Scientific) for 10 min at RT, and incubated with primary antibodies against RIM1 (1:2000, rabbit, Cat. No. 140013, Synaptic Systems) and Flag-tag (1:1000, mouse, Cat. No. F1804, Sigma-Aldrich), or Myc-tag (1:200, mouse, Cat. No. ab32, Abcam) diluted in 0.1% Triton X-100 overnight at 4 °C. .. The next day, cells were washed with DPBS and subsequently incubated with the corresponding fluorescent secondary antibodies (1:400; Alexa Fluor 568 goat anti-rabbit (Cat. No. A11011, Invitrogen) and Alexa Fluor 488 goat anti-mouse (Cat. No. A11001, Invitrogen) were used) diluted in 0.1% Triton X-100 for 2 h at RT.

    SYBR Green Assay:

    Article Title: Polyplex of peptide-mannan and RNA for intranasal delivery of TGF-β siRNA in treatment of pulmonary fibrosis
    Article Snippet: GAPDH reverse and forward primers were purchased from Integrated DNA Technologies, Inc (Coralville, IA). .. Zeba Spin Desalting Columns (7k MOCO), RiboGreen RNA Assay Kit, PowerUp SYBR Green Master Mix, High-Capacity cDNA Reverse Transcription Kit, DMEM/F-12, CHCA MALDI Matrix, and fetal bovine serum (FBS), Alexa Fluor 647 Dye (AF647), phosphate-buffered saline (PBS, pH = 7.4) were purchased from ThermoFisher Scientific (Waltham, MA). .. TGF-β siRNA and Cy5-labeled TGF-β siRNA were purchased from Horizon discovery (Cambridge, UK).

    Reverse Transcription:

    Article Title: Polyplex of peptide-mannan and RNA for intranasal delivery of TGF-β siRNA in treatment of pulmonary fibrosis
    Article Snippet: GAPDH reverse and forward primers were purchased from Integrated DNA Technologies, Inc (Coralville, IA). .. Zeba Spin Desalting Columns (7k MOCO), RiboGreen RNA Assay Kit, PowerUp SYBR Green Master Mix, High-Capacity cDNA Reverse Transcription Kit, DMEM/F-12, CHCA MALDI Matrix, and fetal bovine serum (FBS), Alexa Fluor 647 Dye (AF647), phosphate-buffered saline (PBS, pH = 7.4) were purchased from ThermoFisher Scientific (Waltham, MA). .. TGF-β siRNA and Cy5-labeled TGF-β siRNA were purchased from Horizon discovery (Cambridge, UK).



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    Image Search Results


    Methodological workflow from sample preparation to measuring the cellular response capacity (CRC). Following blood collection, samples are prepared for flow cytometric analysis either unstimulated (PBS as buffer control) or stimulated with an inflammatory cocktail (cocktail of N-formylmethionyl-leucyl-phenylalanine, platelet-activating factor, and tumor necrosis factor). The CRC is calculated as the ratio of median fluorescence intensity (MFI) between stimulated and unstimulated neutrophils. Three approaches, classic, simple, and kinetic CRC, offer distinct advantages and limitations based on technical aspects such as manual processing steps (e.g., centrifugation) and incubation time. n =13–14. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing patients with sepsis at all time points (shown in the figure: 0 h, not shown in the figure: 24, 72, 120 h) with healthy volunteers (HV). P -values are indicated above the respective data points. Asterisks indicate significant differences between HV and patients at 0 h only. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. AUC. Area under the curve; AU. Arbitrary units; PBS. Phosphate-buffered saline; E. coli Escherichia coli .

    Journal: Military Medical Research

    Article Title: The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis

    doi: 10.1016/j.mmr.2026.100010

    Figure Lengend Snippet: Methodological workflow from sample preparation to measuring the cellular response capacity (CRC). Following blood collection, samples are prepared for flow cytometric analysis either unstimulated (PBS as buffer control) or stimulated with an inflammatory cocktail (cocktail of N-formylmethionyl-leucyl-phenylalanine, platelet-activating factor, and tumor necrosis factor). The CRC is calculated as the ratio of median fluorescence intensity (MFI) between stimulated and unstimulated neutrophils. Three approaches, classic, simple, and kinetic CRC, offer distinct advantages and limitations based on technical aspects such as manual processing steps (e.g., centrifugation) and incubation time. n =13–14. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing patients with sepsis at all time points (shown in the figure: 0 h, not shown in the figure: 24, 72, 120 h) with healthy volunteers (HV). P -values are indicated above the respective data points. Asterisks indicate significant differences between HV and patients at 0 h only. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. AUC. Area under the curve; AU. Arbitrary units; PBS. Phosphate-buffered saline; E. coli Escherichia coli .

    Article Snippet: Subsequently, the monovettes were exposed to either phosphate-buffered saline (PBS) with calcium and magnesium (PBS +/+ , #14080055, Gibco, Thermo Fisher Scientific, Waltham, USA) and the bacterial culture media [buffer control of Escherichia coli ( E. coli ) suspension, hereafter referred to as BuC], viable E. coli bacteria (ATCC line 25922, DSMZ, Braunschweig, Germany), or LPS (100 ng/ml, from E. coli O55:B5, #L2637, Sigma Aldrich, Steinheim, Germany).

    Techniques: Sample Prep, Control, Fluorescence, Centrifugation, Incubation, Saline

    Comparison of different approaches to measure the cellular response capacity (CRC) for CD11b on neutrophil granulocytes. Blood from healthy volunteers was incubated with either PBS (Control) or 100 ng/ml LPS for 60 min in the ex vivo whole blood model. a Analysis of the median fluorescence intensity (MFI). b Evaluation of the CRC as determined by different approaches (classic, simple, and kinetic CRC). c, d Change in fluorescence intensity and change in CRC using the kinetic CRC approach, comparing blood with previous exposure to LPS or PBS (buffer control) from the ex vivo whole blood model. In both c and d , the single-cell values measured by flow cytometry were condensed using a moving median with a window of 9 cells. This moving median was then approximated with a 5 th -degree polynomial function. The plots display these polynomial functions together with the baseline (median before stimulation) and a connecting line from the baseline to the polynomial function for 30 s after stimulation. Values are shown as median and interquartile range. n= 10. Statistical analysis was performed using the Mann-Whitney U test. ⁎⁎⁎ P <0.001. AU. Arbitrary units; LPS. Lipopolysaccharide; PBS. Phosphate-buffered saline.

    Journal: Military Medical Research

    Article Title: The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis

    doi: 10.1016/j.mmr.2026.100010

    Figure Lengend Snippet: Comparison of different approaches to measure the cellular response capacity (CRC) for CD11b on neutrophil granulocytes. Blood from healthy volunteers was incubated with either PBS (Control) or 100 ng/ml LPS for 60 min in the ex vivo whole blood model. a Analysis of the median fluorescence intensity (MFI). b Evaluation of the CRC as determined by different approaches (classic, simple, and kinetic CRC). c, d Change in fluorescence intensity and change in CRC using the kinetic CRC approach, comparing blood with previous exposure to LPS or PBS (buffer control) from the ex vivo whole blood model. In both c and d , the single-cell values measured by flow cytometry were condensed using a moving median with a window of 9 cells. This moving median was then approximated with a 5 th -degree polynomial function. The plots display these polynomial functions together with the baseline (median before stimulation) and a connecting line from the baseline to the polynomial function for 30 s after stimulation. Values are shown as median and interquartile range. n= 10. Statistical analysis was performed using the Mann-Whitney U test. ⁎⁎⁎ P <0.001. AU. Arbitrary units; LPS. Lipopolysaccharide; PBS. Phosphate-buffered saline.

    Article Snippet: Subsequently, the monovettes were exposed to either phosphate-buffered saline (PBS) with calcium and magnesium (PBS +/+ , #14080055, Gibco, Thermo Fisher Scientific, Waltham, USA) and the bacterial culture media [buffer control of Escherichia coli ( E. coli ) suspension, hereafter referred to as BuC], viable E. coli bacteria (ATCC line 25922, DSMZ, Braunschweig, Germany), or LPS (100 ng/ml, from E. coli O55:B5, #L2637, Sigma Aldrich, Steinheim, Germany).

    Techniques: Comparison, Incubation, Control, Ex Vivo, Fluorescence, Single Cell, Flow Cytometry, MANN-WHITNEY, Saline

    Characterization of extracellular vesicles (EVs) derived from conditioned medium (CM). (a) Particle size distribution and concentration of EVs measured by nanoparticle tracking analysis. Median particle diameter: 143.6 nm; particle concentration: 1.6 × 10 9 particles/mL per 1 mL of unpurified CM prior to EV isolation. (b) Zeta potential of EVs measured in phosphate-buffered saline, showing a negatively charged surface of −36.38 mV. (c) Quantification of CD63-positive EVs using a Tim4–CD63 sandwich enzyme-linked immunosorbent assay. EV concentration is expressed per 1 mL of pre-purified CM. Statistical analysis using Welch's t -test demonstrated a significant difference between CM and EV samples ( n = 3 per group, ∗ p < 0.001).

    Journal: Regenerative Therapy

    Article Title: Dental pulp cell–derived conditioned culture media promotes periodontal repair through immunomodulation and bone regeneration

    doi: 10.1016/j.reth.2026.101144

    Figure Lengend Snippet: Characterization of extracellular vesicles (EVs) derived from conditioned medium (CM). (a) Particle size distribution and concentration of EVs measured by nanoparticle tracking analysis. Median particle diameter: 143.6 nm; particle concentration: 1.6 × 10 9 particles/mL per 1 mL of unpurified CM prior to EV isolation. (b) Zeta potential of EVs measured in phosphate-buffered saline, showing a negatively charged surface of −36.38 mV. (c) Quantification of CD63-positive EVs using a Tim4–CD63 sandwich enzyme-linked immunosorbent assay. EV concentration is expressed per 1 mL of pre-purified CM. Statistical analysis using Welch's t -test demonstrated a significant difference between CM and EV samples ( n = 3 per group, ∗ p < 0.001).

    Article Snippet: For subsequent passaging, the medium was removed and the dishes were rinsed twice with phosphate-buffered saline (Nacalai Tesque).

    Techniques: Derivative Assay, Concentration Assay, Isolation, Zeta Potential Analyzer, Saline, Sandwich ELISA, Purification